Chemical Biology: A Practical Course

The present selection of 12 inspiring experiments has been carefully selected to cover the most commonly used laboratory techniques for the synthesis and analysis of bioactive compounds. Together, they provide a solid introduction to the skills and techniques that are required for the successful study of Chemical Biology..
Here you will learn how to

• synthesize a peptide by solid phase synthesis
• modify a protein by covalent attachment of a lipid chain
• modify an oligonucleotide and use it for DNA-directed immobilization
• isolate an enzyme for in vitro synthesis of a polysaccharide
• design a ligand for a protein by molecular modeling
• perform a proteome analysis in yeast

All experiments haven been thoroughly tested in the authors' laboratory and come with an easy to follow step-by-step instruction, complemented by abundant practical hints and by a thorough treatment of the underlying theory and mechanisms.

Take your first steps in combinatorial synthesis or synthesize bioactive molecules such as antibiotics. Search for mutations in DNA using chemical probes or perform a proteome analysis in yeast.
The present selection of 12 inspiring experiments is tailored for maximum learning effect at minimal expense of time and equipment. Almost all currently used laboratory techniques in synthesis and analysis of bioactive compounds are represented at least once.
Abundant practical hints are complemented by a thorough treatment of the underlying theory and mechanisms.