Molecular Biology

MOLECULAR BIOLOGY (Principles and Practices) introduces undergraduates, postgraduates as well as research students of biotechnology to basic experimental molecular biology laboratory techniques. Emphasis has been given to techniques encountered in practical classes with the principles and theories behind this explained in detail. No contemporary book on Practical Molecular Biology would be complete without chapters on Bio-Safety, Laboratory Set-up, Molecular Biology Calculations and Glossary. These topics have been extensively covered in this book. This is essential reading for all biosciences students, biotechnology and preclinical medical students for whom practical molecular biology forms part of the syllabus.
Table Of Contents :
UNIT I : MOLECULAR BIOLOGY LABORATORY-AN INTRODUCTION
Part 1 - General Laboratory Facilities
Part 2 - General Laboratory Practices
Part 3 - Laboratory Safety Guidelines and Health Hazards

UNIT II : CALCULATIONS USED IN MOLECULAR BIOLOGY
Part 1 - Calculations used in Molecular Biology

UNIT III : DNA ISOLATION AND PURIFICATION
Part 1 - Plasmid DNA
Part 2 - Bacterial (E.Coli) DNA
Part 3 - Plant Genomic DNA
Part 4 - Animal Genomic DNA

UNIT IV : ELECTROPHORESIS
Part 1 - A General Account
Part 2 - Agarose Gel Electrophoresis of DNA
Part 3 - Polyacrylamide Gel Electrophoresis of DNA
Part 4 - Polyacrylamide Gel Electrophoresis of Proteins

UNIT V : CHECKING QUALITY AND QUANTITY OF DNA
Part 1 - Spectrophotometric Method
Part 2 - Electrophoretic Method

UNIT VI : RESTRICATION ENDONUCLEASES
Part 1 - A General Account
Part 2 - Restriction Enzyme Digestion and Size Determination of  DNA Fragments.
Part 3 - Restriction Mapping of Lambda DNA with three Enzymes viz Eco RI, Bam HI and Hind III

UNIT VII : LIGATION
Part 1 - A General Account
Part 2 - Ligation of a DNA Fragment, Carrying Gene of Interest, to a Plasmid for Constructing a Recombinant DNA Molecule

UNIT VIII : TRANSFORMATION
Part 1 - Transformation a General Account
Part 2 - Bacterial Plasmid Transformation
Part 3 - Agrobacterium-Mediated Transformation in Plants
            (e.g., Nicotiana Tabacum)

UNIT IX : POLYMERASE CHAIN REACTION
Part 1 - PCR-a General Account
Part 2 - Performing Amplification of DNA using PCR in a Routine Laboratory Experiment

UNIT X : MOLECULAR MARKERS
Part 1 - Molecular Markers-a General Account
Part 2 - RFLP (Restriction Fragment Length Polymorphism)
Part 3 - RAPD (Random amplified polymorphic DNA)
Part 4 - AFLP (Amplified Fragment Length Polymorphism)
Part 5 - Simple Sequence Repeats (SSRs) or Micro Satellites

UNIT XI : MOLECULAR MARKERS AND MAPPING
Part 1 - Molecular Markers and Mapping

UNIT XII : DNA SEQUENCING
Part 1 - A General Account
Part 2 - Performing DNA Sequencing in Laboratory by the Sanger's Dideoxy Method using Sequenase Enzyme

UNIT XIII : IN VITRO MUTAGENESIS
Part 1 - A General Account
Part 2 - Performing Oligonucleotide Directed Mutagenesis of Single Stranded DNA

MOLECULAR BIOLOGY GLOSSARY
SOURCES AND SUGGESTED READING
IMPORTANT WEBSITES

ANNEXURE  I     : Preparation of Cleansing solution, Acid and Albati (1 N) solution, Standard buffer solutions
ANNEXURE  Il    : Molecular Biology Laboratory Waste Disposal
ANNEXURE  IlI   : Hazard Symbols and Precautions
ANNEXURE  IV   : Inactivation of Ethidium Bromide
ANNEXURE  V    : Biological Agents Causing Biohazards
ANNEXURE  Vl   : Risk Groups
ANNEXURE  VIl  : Biosafety Committees in India
ANNEXURE  VIIl : Preparation of Buffer Equilibrated Phenol
ANNEXURE  IX   : Miscellaneous Information
ANNEXURE  X    : Periodic Table of the Elements
About Author :
Dr. Priyanka Siwach is a budding molecular biologist having published many research papers, review articles on plant biotechnology and molecular biology in various Indian and foreign journals. She obtained her Master's degree in Biotechnology and Molecular Biology in the year 1998 and Ph.D. in the same subject in 2001 from Ch. Charan Singh Haryana Agricultural University, Hisar. She was awarded "Silver Jubilee Gold Medal for Women Scientist" for the year 2001 for her eminent research work in Ph.D. programme. She has been teaching as Lecturer in the Department of Biotechnology, Guru Jambheshewar University, Hisar since 2001. During her teaching career of nearly six years, she has guided a large number of research students in their M.Sc. dissertation, six M. Phil. students and two Ph. D. students. Dr. Priyanka has earned one major research proposal as Principal Investigator and another as Co-Principal Investigator from UGC during this short span of nearly six years. She has participated in many national and international conferences and has also delivered lectures at many degree colleges. At present she is on deputation in Ch. Devi Lal University, Sirsa.

Dr. Namita Singh is a senior Lecturer in the Department of Biotechnology at Guru Jambheshewar University of Science and Technology, Hisar, Haryana. She has done her Ph.D. from the Institute of Microbiology and Biotechnology, Barkatullaha University, Bhopal. She is recipient of many national and international Research awards and fellowships (UGC-JRF, SRF, UNESCO-American Society for Microbiology fellowship (USA) and Blaustein fellowship (Isreal). She has published many research articles/papers in national and international journals.UMB-9399